略歴

2003年3月 京都大学理学部 卒業
2008年3月 京都大学大学院理学研究科博士後期課程 修了
2008年4月 千葉大学大学院融合科学研究科 学術研究支援員
2010年4月 カリフォルニア大学バークレー校化学科 JSPS海外特別研究員
2012年4月 理化学研究所 基礎科学特別研究員
2015年4月 理化学研究所 光量子工学領域 研究員
2015年5月 佐賀大学大学院工学系研究科 循環物質化学専攻 助教
2016年2月 佐賀大学大学院工学系研究科 先端融合工学専攻 特任講師
2018年4月 佐賀大学大学院工学系研究科 先端融合工学専攻 准教授
2019年4月 佐賀大学理工学部・化学部門 准教授

研究テーマ

光受容タンパク質の反応機構解明
ラマン光学活性を用いたタンパク質活性部位の計測

研究業績

(学術雑誌に発表した論文)

(29) T. Fujisawa*, H. Kiyota, T. Kikukawa, M. Unno* "Low-temperature Raman spectroscopy of halorhodopsin from Natronomonas pharaonis: Structural discrimination of blue-shifted and red-shifted photoproducts"
Biochemistry 58, 4159-4167, 2019.

(28) A. Iizuka, K. Kajimoto, T. Fujisawa, T. Tsukamoto, T. Aizawa, N. Kamo, K.-H. Jung, M. Unno, M. Demura, T. Kikukawa* "Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin"
Scientific Reports 9, 10711, 2019.

(27) S. Osoegawa, R. Miyoshi, K. Watanabe, Y. Hirose, T. Fujisawa, M. Ikeuchi,M. Unno* "Identification of the deprotonated pyrrole nitrogen of the bilin-based photoreceptor by Raman spectroscopy with an advanced computational analysis"
Journal of Physical Chemistry B, 123, 3242-3247, 2019.

(26) Y. Takaoka, S. Esaki, K. Sakaguchi, T. Fujisawa, M. Unno, S. Morisada, K. Ohto, H. Kawakita*, "Size-dependent separation of graphene oxide by deformation of packed-gel in a chromatographic column"
Separation Science and Technology, https://doi.org/10.1080/01496395.2019.1586726

(25)T. Fujisawa*, M. Abe, J. Tamogami, T. Kikukawa, N. Kamo, M. Unno*, "Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin"
FEBS Letters, 592, 3054-3061, 2018.

(24) S. Haraguchi, T. Shingae, T. Fujisawa, N. Kasai, T. Hanamoto, W. D. Hoff, M. Unno*, "Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration"
Proceedings of the National Academy of Sciences of USA, 115, 8671-8675, 2018.

(23)T. Fujisawa*, S. Masuda*, "Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins"
Biophysical Reviews, 2017.

(22) W. Piao, K. Hanaoka*, T. Fujisawa, S. Takeuchi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, T. Nagano, Y. Urano*,"Development of an Azo-based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy"
Journal of the American Chemical Society, 139, 13713-13719, 2017.

(21)T. Fujisawa*, R. L. Leverenz, M. Nagamine, C A. Kerfeld, M. Unno*,
"Raman Optical Activity Reveals Carotenoid Photoactivation Events in the Orange Carotenoid Protein in Solution"
Journal of the American Chemical Society, 139, 10456-10460, 2017.

(20) K. Kajimoto, T. Kikukawa, H. Nakashima, H. Yamaryo, Y. Saito, T. Fujisawa, M. Demura, M. Unno*, "Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus"
Journal of Physical Chemistry B, 121, 4431-4437, 2017.

(19) H. Kuramochi, T. Fujisawa, S. Takeuchi, T. Tahara*, "Broadband Stimulated Raman Spectroscopy in the Deep Ultraviolet Region"
Chemical Physics Letters, 683, 543-546, 2017.

(18) N. Shin, K. Hanaoka*, W. Piao, T. Miyakawa, T. Fujisawa, S. Takeuchi, S. Takahashi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, M. Tanokura, T. Nagano, Y. Urano*, "Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates", ACS Chemical Biology, 12, 558-563, 2017.

(17) M. Furuta, T. Fujisawa, H. Urago, T. Eguchi, T. Shingae, S. Takahashi, E. W. Blanch, M. Unno*, "Raman Optical Activity of Tetra-alanine in the Poly(L-proline) II Type Peptide Conformation"
Physical Chemistry Chemical Physics, 19, 2078-2086, 2016.

(16)藤澤知績、 海野雅司
「ラマン光学活性分光法」
分光研究、第65巻、第4号(2016)218-231.

(15) H. Shirota*, S. Kakinuma, K. Takahashi, A. Tago, H. Jeong, T. Fujisawa
"Ultrafast Dynamics in Aromatic Cation Based Ionic Liquids: A Femtosecond Raman-Induced Kerr Effect Spectroscopic Study"
Bulletin of the Chemical Society of Japan, 89, 1106-1128, 2016.

(14) T. Fujisawa, H. Kuramochi, H. Hosoi, S. Takeuchi, T. Tahara*
"Role of Coherent Low-Frequency Motion in Excited-State Proton Transfer of Green Fluorescent Protein Studied by Time-Resolved Impulsive Stimulated Raman Spectroscopy"
Journal of the American Chemical Society, 138, 3942-3945, 2016.

(13) T. Fujisawa, S. Takeuchi, S. Masuda, T. Tahara*
"Signaling-state formation mechanism of a BLUF protein PapB from the purple bacterium Rhodopseudomonas palustris studied by femtosecond time-resolved absorption Spectroscopy"
Journal of Physical Chemistry B, 118, 14761-14773, 2014.

(12) T. Fujisawa, M. Creelman, R. A. Mathies*
"Structural dynamics of a non-covalent charge transfer complex from femtosecond stimulated Raman spectroscopy"
Journal of Physical Chemistry B, 116, 10453-10460, 2012.

(11) H. Shirota*, H. Fukazawa, T. Fujisawa, J. F. Wishart
"Heavy atom substitution effects in non-aromatic ionic liquids: Ultrafast dynamics and physical properties"
Journal of Physical Chemistry B, 114, 9400-9412, 2010.

(10) T. Fujisawa, K. Nishikawa, H. Shirota*
"Comparison of interionic/intermolecular vibrational dynamics between ionic liquids and concentrated electrolyte solutions"
Journal of Chemical Physics, 131, 244519, 2009.

(9) H. Shirota*, T. Fujisawa, H. Fukazawa, K. Nishikawa
"Ultrafast dynamics in aprotic molecular liquids: A femtosecond Raman-induced Kerr effect spectroscopic study"
Bulletin of the Chemical Society of Japan, 82, 1347-1366, 2009.

(8) K. Osawa, T. Hamamoto, T. Fujisawa, M. Terazima, H. Sato, Y. Kimura*
"Raman spectroscopic study on solvation of p-aminobenzonitrile in supercritical water and methanol"
Journal of Physical Chemistry A, 113, 3143-3154, 2008.

(7) T. Fujisawa*, M. Terazima, Y. Kimura*
"Solvent effects on the local structure of p-nitroaniline in supercritical water and supercritical alcohols"
Journal of Physical Chemistry A, 112, 5515-5526, 2008.

(6) T. Fujisawa, T. Ito, M. Terazima, Y. Kimura*
"Raman spectroscopic study of the solvation of decafluorobenzophenone ketyl radical and related compounds in 2-propanol at ambient to supercritical temperatures"
Journal of Physical Chemistry A, 112, 1914-1921, 2008.

(5) T. Fujisawa, M. Terazima, Y. Kimura*, M. Maroncelli
"Resonance Raman study of the solvation of p-nitroaniline in supercritical water"
Chemical Physics Letters, 430, 303-308, 2006.

(4) T. Fujisawa, M. Terazima, Y. Kimura*
"Excitation wavelength dependence of the Raman Stokes shift of N,N-dimethyl-p-nitroaniline"
Journal of Chemical Physics, 124, 184503 (1-9), 2006.

(3) T. Fujisawa, M. Fukuda, M. Terazima, Y. Kimura*
"Raman spectroscopic study on solvation of diphenylcyclopropenone and phenol blue in room temperature ionic liquids"
Journal of Physical Chemistry A, 110, 6164-6172, 2006.

(2) 木村佳文、網田 富士嗣、藤澤知績
「超臨界流体中における非線形レーザー分光」
高圧力の科学と技術、日本高圧力学会、16巻2号、87-94、2006.

(1) Y. Kimura*, M. Fukuda, T. Fujisawa, M. Terazima
"Acceptor number of room temperature ionic liquid determined by the Raman spectrum of diphenylcyclopropenone"
Chemistry Letters, 34, 338-339, 2005.


(国際会議プロシーディング)
(2) T. Fujisawa, H. Kuramochi, S. Takeuchi, T. Tahara “Time-Resolved Impulsive Raman Study of Excited State Structures of Green Fluorescent Protein”
Ultrafast Phenomena XIX , Proceedings of the 19th International Conference, 539-542, 2014.

(1) T. Fujisawa, E. Maru , F. Amita, M. Harada, T. Uruga, and Y. Kimura
"Development and Application of the Multipurpose Optical Flow Cell under Supercritical Condition of Water" Proceedings of the 14th International Conference on the Properties of Water and Steam, 445-450, 2005.