略歴

2003年3月　京都大学理学部　卒業
2008年3月　京都大学大学院理学研究科博士後期課程　修了
2008年4月　千葉大学大学院融合科学研究科　学術研究支援員
2010年4月　カリフォルニア大学バークレー校化学科　JSPS海外特別研究員
2012年4月　理化学研究所　基礎科学特別研究員
2015年4月　理化学研究所　光量子工学領域　研究員
2015年5月　佐賀大学大学院工学系研究科　循環物質化学専攻　助教
2016年2月　佐賀大学大学院工学系研究科　先端融合工学専攻　特任講師
2018年4月　佐賀大学大学院工学系研究科　先端融合工学専攻　准教授
2019年4月　佐賀大学理工学部・化学部門　准教授

研究テーマ

光受容タンパク質の反応機構解明
ラマン光学活性を用いたタンパク質活性部位の計測

研究業績

(原著論文)
(48) T. Fujisawa*, N. Tanaka, J. Tamogami, M. Unno, "Retinal Chromophore Configuration in the O Intermediate of Sensory Rhodopsin II from Natronomonas pharaonis", Biochemistry, in press.

(47) K. Miyazaki, T. Kikukawa, M. Unno,T. Fujisawa*, "Chromophore Structural Change during the Photocycle of a Light-Driven Cl- Pump from Mastigocladopsis repens: A Cryogenic Raman Study", Journal of Physical Chemistry B, 128, 9692-9698, 2024.

(46) T. Kamo, T. Matsushita, M. Hamada,T. Fujisawa, T. Eki, M. Unno, Y. Hirose*, "Probing bilin-protein interaction in the protochromic photocycle of cyanobacteriochrome RcaE by site-directed mutagenesis", Plant Cell Physiology, pcae085, 2024.

(45) T. Nagae, Y. Fujita, T. Tsuchida, T. Kamo, R. Seto, M. Hamada, H. Aoyama, A. Sato-Tomita, T. Fujisawa, T. Eki, Y. Miyanoiri, Y. Ito, T. Soeta, Y. Ukaji, M. Unno*, M. Mishima*, Y. Hirose*, "Green/red light-sensing mechanism in the chromatic acclimation photosensor", Science Advances. 10, eadn8386, 2024.

(44) E. Machalska*, M. Halat, T. Tani, T. Fujisawa, M. Unno, A. Kudelski, M. Baranska, G. Zajac*, "Why Does One Measure Resonance Raman Optical Activity? A Unique Case of Measurements under Strong Resonance versus Far-from-Resonance Conditions", The Journal of Physical Chemistry Letters, 15, 4913-4919, 2024.

(43) T. Fujisawa*, K. Kinoue, R. Seike, T. Kikukawa, M. Unno, "Configurational Changes of Retinal Schiff Base during Membrane Na+ Transport by a Sodium Pumping Rhodopsin", The Journal of Physical Chemistry Letters, 15, 1993-1998, 2024.

(42) T. Wu, P. Bour*, T. Fujisawa, M. Unno*, "Molecular Vibrations in Chiral Europium Complexes Revealed by Near-Infrared Raman Optical Activity", Advanced Science, 11, 2305521, 2024.

(41) T. Fujisawa, T. Shingae, J. Ren, S. Haraguchi, T. Hanamoto, W. D. Hoff, M. Unno*, "Spectroscopic Validation of Crystallographic Structures of a Protein Active Site by Chiroptical Spectroscopy", The Journal of Physical Chemistry Letters, 14, 9304-9309, 2023.

(40) K. Nishikawa, R. Kuroiwa, J. Tamogami, M. Unno*, T. Fujisawa*, "Raman Optical Activity of Retinal Chromophore in Sensory Rhodopsin II" Journal of Physical Chemistry B, 127, 7244-7250, 2023.

(39) M. Ohya, T. Kikukawa, J. Matsuo, T. Tsukamoto, R. Nagaura, T. Fujisawa, M. Unno*, "Structure and Heterogeneity of Retinal Chromophore in Chloride Pump Rhodopsins Revealed by Raman Optical Activity" Journal of Physical Chemistry B, 127, 4775-4782, 2023.

(38) T. Fujisawa, T. Kinoue, R. Seike, T. Kikukawa, M. Unno*, "Reisomerization of Retinal Represents a Molecular Switch Mediating Na+ Uptake and Release by a Bacterial Sodium-pumping Rhodopsin" Journal of Biologial Chemistry, 296, 100792, 2022.

(37) Y. Okuda, R. Miyoshi, T. Kamo, T. Fujisawa, T. Nagae, M. Mishima, T. Eki, Y. Hirose*, M. Unno*, "Raman Spectroscopy of an Atypical C15-E,syn Bilin Chromophore in Cyanobacteriochrome RcaE" Journal of Physical Chemistry B, 126, 813-821, 2022.

(36) T. Fujisawa, S. Masuda, S. Takeuchi, T. Tahara*, "Femtosecond Time-Resolved Absorption Study of Signaling State of a BLUF Protein PixD from the Cyanobacterium Synechocystis: Hydrogen-Bond Rearrangement Completes during Forward Proton-Coupled Electron Transfer"
Journal of Physical Chemistry B, 125, 12154-12165, 2021.

(35) T. Fujisawa*, K. Nishikawa, J. Tamogami, M. Unno*, "Conformational analysis of a retinal Schiff base chromophore in proteorhodopsin by Raman optical activity", The Journal of Physical Chemistry Letters, 12, 9564-9568, 2021.

(34) M. Unno*, Y. Hirose, M. Mishima, T. Kikukawa, T. Fujisawa, J. Tamogami, "Spectroscopic approach for exploring structure and function of photoreceptor proteins", Biophysics and Physicobiology, 18, 127-130, 2021.

(33) T. Nagae, M. Unno*, T. Koizumi, Y. Miyanoiri, T. Fujisawa, K. Masui, T. Kamo, K. Wada, T. Eki, Y. Ito, Y. Hirose*, M. Mishima*, "Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE"
Proceedings of the National Academy of Sciences of the USA, 118, e2024583118, 2021.

(32) S. Kamura, T. Tani, H. Matsuo, Y. Onaka, T. Fujisawa, M. Unno*, "New Probe for Porcelain Glazes by Luminescence at Near-Infrared Excitation"
ACS Omega, 6, 7829-7833, 2021.

(31) T. Akatsu*, T. Fujisawa, M. Unno "Prestress in alumina-strengthened porcelain as estimated with Raman scattering spectroscopy"
Journal of the Ceramic Society of Japan, 129, 267-270, 2021.

(30) Y. Nakamizo, T. Fujisawa*, T. Kikukawa, A. Okamura, H. Baba, M. Unno* "Low-temperature Raman spectroscopy of sodium-pump rhodopsin from Indibacter alkaliphilus: Insight of Na+ binding for active Na+ transport"
Physical Chemistry Chemical Physics, 23, 2072-2079, 2021.

(29) J. Matsuo, T. Kikukawa, T. Fujisawa, W. D. Hoff, M. Unno* "Watching a Molecular Twist in a Protein by Raman Optical Activity"
The Journal of Physical Chemistry Letters, 11, 8579-8584, 2020.

(28) T. Sato, T. Kikukawa, R. Miyoshi, K. Kajimoto, C. Yonekawa, T. Fujisawa, M. Unno, T. Eki, Y. Hirose*, "Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome"
Journal of Biological Chemistry, 294, 18909-18922, 2019.

(27) T. Fujisawa*, H. Kiyota, T. Kikukawa, M. Unno* "Low-temperature Raman spectroscopy of halorhodopsin from Natronomonas pharaonis: Structural discrimination of blue-shifted and red-shifted photoproducts"
Biochemistry, 58, 4159-4167, 2019.

(26) A. Iizuka, K. Kajimoto, T. Fujisawa, T. Tsukamoto, T. Aizawa, N. Kamo, K.-H. Jung, M. Unno, M. Demura, T. Kikukawa* "Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin"
Scientific Reports, 9, 10711, 2019.

(25) S. Osoegawa, R. Miyoshi, K. Watanabe, Y. Hirose, T. Fujisawa, M. Ikeuchi,M. Unno* "Identification of the deprotonated pyrrole nitrogen of the bilin-based photoreceptor by Raman spectroscopy with an advanced computational analysis"
Journal of Physical Chemistry B, 123, 3242-3247, 2019.

(24) Y. Takaoka, S. Esaki, K. Sakaguchi, T. Fujisawa, M. Unno, S. Morisada, K. Ohto, H. Kawakita*, "Size-dependent separation of graphene oxide by deformation of packed-gel in a chromatographic column"
Separation Science and Technology, 55, 1356-1363, 2020.

(23)T. Fujisawa*, M. Abe, J. Tamogami, T. Kikukawa, N. Kamo, M. Unno*, "Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin"
FEBS Letters, 592, 3054-3061, 2018.

(22) S. Haraguchi, T. Shingae, T. Fujisawa, N. Kasai, T. Hanamoto, W. D. Hoff, M. Unno*, "Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration"
Proceedings of the National Academy of Sciences of the USA, 115, 8671-8675, 2018.

(21)T. Fujisawa*, S. Masuda*, "Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins"
Biophysical Reviews, 2017.

(20) W. Piao, K. Hanaoka*, T. Fujisawa, S. Takeuchi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, T. Nagano, Y. Urano*,"Development of an Azo-based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy"
Journal of the American Chemical Society, 139, 13713-13719, 2017.

(19)T. Fujisawa*, R. L. Leverenz, M. Nagamine, C A. Kerfeld, M. Unno*,
"Raman Optical Activity Reveals Carotenoid Photoactivation Events in the Orange Carotenoid Protein in Solution"
Journal of the American Chemical Society, 139, 10456-10460, 2017.

(18) K. Kajimoto, T. Kikukawa, H. Nakashima, H. Yamaryo, Y. Saito, T. Fujisawa, M. Demura, M. Unno*, "Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus"
Journal of Physical Chemistry B, 121, 4431-4437, 2017.

(17) H. Kuramochi, T. Fujisawa, S. Takeuchi, T. Tahara*, "Broadband Stimulated Raman Spectroscopy in the Deep Ultraviolet Region"
Chemical Physics Letters, 683, 543-546, 2017.

(16) N. Shin, K. Hanaoka*, W. Piao, T. Miyakawa, T. Fujisawa, S. Takeuchi, S. Takahashi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, M. Tanokura, T. Nagano, Y. Urano*, "Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates", ACS Chemical Biology, 12, 558-563, 2017.

(15) M. Furuta, T. Fujisawa, H. Urago, T. Eguchi, T. Shingae, S. Takahashi, E. W. Blanch, M. Unno*, "Raman Optical Activity of Tetra-alanine in the Poly(L-proline) II Type Peptide Conformation"
Physical Chemistry Chemical Physics, 19, 2078-2086, 2016.

(14) H. Shirota*, S. Kakinuma, K. Takahashi, A. Tago, H. Jeong, T. Fujisawa
"Ultrafast Dynamics in Aromatic Cation Based Ionic Liquids: A Femtosecond Raman-Induced Kerr Effect Spectroscopic Study"
Bulletin of the Chemical Society of Japan, 89, 1106-1128, 2016.

(13) T. Fujisawa, H. Kuramochi, H. Hosoi, S. Takeuchi, T. Tahara*
"Role of Coherent Low-Frequency Motion in Excited-State Proton Transfer of Green Fluorescent Protein Studied by Time-Resolved Impulsive Stimulated Raman Spectroscopy"
Journal of the American Chemical Society, 138, 3942-3945, 2016.

(12) T. Fujisawa, S. Takeuchi, S. Masuda, T. Tahara*
"Signaling-state formation mechanism of a BLUF protein PapB from the purple bacterium Rhodopseudomonas palustris studied by femtosecond time-resolved absorption Spectroscopy"
Journal of Physical Chemistry B, 118, 14761-14773, 2014.

(11) T. Fujisawa, M. Creelman, R. A. Mathies*
"Structural dynamics of a non-covalent charge transfer complex from femtosecond stimulated Raman spectroscopy"
Journal of Physical Chemistry B, 116, 10453-10460, 2012.

(10) H. Shirota*, H. Fukazawa, T. Fujisawa, J. F. Wishart
"Heavy atom substitution effects in non-aromatic ionic liquids: Ultrafast dynamics and physical properties"
Journal of Physical Chemistry B, 114, 9400-9412, 2010.

(9) T. Fujisawa, K. Nishikawa, H. Shirota*
"Comparison of interionic/intermolecular vibrational dynamics between ionic liquids and concentrated electrolyte solutions"
Journal of Chemical Physics, 131, 244519, 2009.

(8) H. Shirota*, T. Fujisawa, H. Fukazawa, K. Nishikawa
"Ultrafast dynamics in aprotic molecular liquids: A femtosecond Raman-induced Kerr effect spectroscopic study"
Bulletin of the Chemical Society of Japan, 82, 1347-1366, 2009.

(7) K. Osawa, T. Hamamoto, T. Fujisawa, M. Terazima, H. Sato, Y. Kimura*
"Raman spectroscopic study on solvation of p-aminobenzonitrile in supercritical water and methanol"
Journal of Physical Chemistry A, 113, 3143-3154, 2008.

(6) T. Fujisawa*, M. Terazima, Y. Kimura*
"Solvent effects on the local structure of p-nitroaniline in supercritical water and supercritical alcohols"
Journal of Physical Chemistry A, 112, 5515-5526, 2008.

(5) T. Fujisawa, T. Ito, M. Terazima, Y. Kimura*
"Raman spectroscopic study of the solvation of decafluorobenzophenone ketyl radical and related compounds in 2-propanol at ambient to supercritical temperatures"
Journal of Physical Chemistry A, 112, 1914-1921, 2008.

(4) T. Fujisawa, M. Terazima, Y. Kimura*, M. Maroncelli
"Resonance Raman study of the solvation of p-nitroaniline in supercritical water"
Chemical Physics Letters, 430, 303-308, 2006.

(3) T. Fujisawa, M. Terazima, Y. Kimura*
"Excitation wavelength dependence of the Raman Stokes shift of N,N-dimethyl-p-nitroaniline"
Journal of Chemical Physics, 124, 184503 (1-9), 2006.

(2) T. Fujisawa, M. Fukuda, M. Terazima, Y. Kimura*
"Raman spectroscopic study on solvation of diphenylcyclopropenone and phenol blue in room temperature ionic liquids"
Journal of Physical Chemistry A, 110, 6164-6172, 2006.

(1) Y. Kimura*, M. Fukuda, T. Fujisawa, M. Terazima
"Acceptor number of room temperature ionic liquid determined by the Raman spectrum of diphenylcyclopropenone"
Chemistry Letters, 34, 338-339, 2005.


（学術記事、著書など）
(10)T. Fujisawa*, M. Unno*, "Near-Infrared Excited Raman Optical Activity as a Tool to Uncover Active Sites of Photoreceptor Proteins", Journal of Physical Chemistry B, 128, 2228-2235, 2024.

(9)藤澤知績、 海野雅司
「ラマン光学活性によるプロテオロドプシン中のレチナール色素の構造解析」
生物物理、第62巻、（2022）331-333.

(8)藤澤知績、 海野雅司
「近赤外ラマン分光法の色素タンパク質への応用」
分光研究、第71巻、第4号（2022）105-107.

(7)T. Fujisawa, M. Unno, "Advanced spectral analysis of complex molecular system" In Molecular and Laser Spectroscopy: Advances and Applications: Volume 3; Gupta, V. P. Ed.; Elsevier Inc.; pp 25-54 (2022)

(6)M. Unno, Y. Hirose, M. Mishima, T. Kikukawa, T. Fujisawa, T. Iwata, J. Tamogami, "Spectroscopic approach for exploring structure and function of photoreceptor proteins", Biophysics and Physicobiology, 18, 127-130, 2021.

(5) T. Fujisawa, M. Unno "Vibrational optical activity spectroscopy"
Chapter 2 in Molecular and Laser Spectroscopy: Advances and Applications: Volume 2; Gupta, V. P. & Ozaki, Y. Eds.; Elsevier Inc.; pp 41-82 (2020)

(4) T. Fujisawa, "Raman Optical Activity of Proteins"
Encyclopedia of Analytical Chemistry, 2020 John Wiley & Sons, Ltd.

(3) T. Fujisawa, S. Masuda "Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins"
Biophysical Revies, 10, 327-337, 2018.

(2)藤澤知績、 海野雅司
「ラマン光学活性分光法」
分光研究、第65巻、第4号（2016）218-231.

(1) 木村佳文、網田　富士嗣、藤澤知績
「超臨界流体中における非線形レーザー分光」
高圧力の科学と技術、日本高圧力学会、16巻2号、（2006）87-94.


（国際会議プロシーディング）
(2) T. Fujisawa, H. Kuramochi, S. Takeuchi, T. Tahara “Time-Resolved Impulsive Raman Study of Excited State Structures of Green Fluorescent Protein”
Ultrafast Phenomena XIX , Proceedings of the 19th International Conference, 539-542, 2014.

(1) T. Fujisawa, E. Maru , F. Amita, M. Harada, T. Uruga, and Y. Kimura
"Development and Application of the Multipurpose Optical Flow Cell under Supercritical Condition of Water" Proceedings of the 14th International Conference on the Properties of Water and Steam, 445-450, 2005.