略歴

Mar. 2003 B.Sc., Faculty of Science, Kyoto University
Mar. 2008 Ph.D., Graduate School of Science, Kyoto University
Apr. 2008 Postdoc, Chiba Unversity
Apr. 2010 JSPS postdoctoral fellow for research abroad, University of California, Berkeley
Apr. 2012 Special Postdoctoral researcher, RIKEN
Apr. 2015 Postdoc, RIKEN
May 2015 Assistant professor, Saga University
Feb. 2016 Lecturer, Saga University
Apr. 2018 Associate professor, Saga University

Research Projects

1. Reaction mechanism in photoreceptor proteins
2. Application of Raman optical activity for 3D structural analysis of photoreceptor proteins

Publications


(29) T. Fujisawa*, H. Kiyota, T. Kikukawa, M. Unno* "Low-temperature Raman spectroscopy of halorhodopsin from Natronomonas pharaonis: Structural discrimination of blue-shifted and red-shifted photoproducts"
Biochemistry 58, 4159-4167, 2019.

(28) A. Iizuka, K. Kajimoto, T. Fujisawa, T. Tsukamoto, T. Aizawa, N. Kamo, K.-H. Jung, M. Unno, M. Demura, T. Kikukawa* "Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin"
Scientific Reports 9, 10711, 2019.

(27) S. Osoegawa, R. Miyoshi, K. Watanabe, Y. Hirose, T. Fujisawa, M. Ikeuchi,M. Unno* "Identification of the deprotonated pyrrole nitrogen of the bilin-based photoreceptor by Raman spectroscopy with an advanced computational analysis"
Journal of Physical Chemistry B, 123, 3242-3247, 2019.

(26) Y. Takaoka, S. Esaki, K. Sakaguchi, T. Fujisawa, M. Unno, S. Morisada, K. Ohto, H. Kawakita*, "Size-dependent separation of graphene oxide by deformation of packed-gel in a chromatographic column"
Separation Science and Technology, https://doi.org/10.1080/01496395.2019.1586726

(25)T. Fujisawa*, M. Abe, J. Tamogami, T. Kikukawa, N. Kamo, M. Unno*, "Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin"
FEBS Letters, https://doi.org/10.1002/1873-3468.13219

(24)S. Haraguchi, T. Shingae, T. Fujisawa, N. Kasai, T. Hanamoto, W. D. Hoff, M. Unno*, "Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration"
Proceedings of the National Academy of Sciences of USA, 115, 8671-8675, 2018.

(23)T. Fujisawa*, S. Masuda*, "Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins"
Biophysical Reviews, 2017.

(22)W. Piao, K. Hanaoka*, T. Fujisawa, S. Takeuchi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, T. Nagano, Y. Urano*,"Development of an Azo-based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy"
Journal of the American Chemical Society, 139, 13713-13719, 2017.

(21)T. Fujisawa*, R. L. Leverenz, M. Nagamine, C A. Kerfeld, M. Unno*,
"Raman Optical Activity Reveals Carotenoid Photoactivation Events in the Orange Carotenoid Protein in Solution"
Journal of the American Chemical Society, 139, 10456-10460, 2017.

(20) K. Kajimoto, T. Kikukawa, H. Nakashima, H. Yamaryo, Y. Saito, T. Fujisawa, M. Demura, M. Unno*, "Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus"
Journal of Physical Chemistry B, 121, 4431-4437, 2017.

(19) H. Kuramochi, T. Fujisawa, S. Takeuchi, T. Tahara*, "Broadband Stimulated Raman Spectroscopy in the Deep Ultraviolet Region"
Chemical Physics Letters, 683, 543-546, 2017.

(18) N. Shin, K. Hanaoka*, W. Piao, T. Miyakawa, T. Fujisawa, S. Takeuchi, S. Takahashi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, M. Tanokura, T. Nagano, Y. Urano*, "Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates", ACS Chemical Biology, 12, 558-563, 2017.

(17) M. Furuta, T. Fujisawa, H. Urago, T. Eguchi, T. Shingae, S. Takahashi, E. W. Blanch, M. Unno*, "Raman Optical Activity of Tetra-alanine in the Poly(L-proline) II Type Peptide Conformation"
Physical Chemistry Chemical Physics, 19, 2078-2086, 2016.

(16)藤澤知績、 海野雅司
「ラマン光学活性分光法」
分光研究、第65巻、第4号(2016)218-231.

(15) H. Shirota*, S. Kakinuma, K. Takahashi, A. Tago, H. Jeong, T. Fujisawa
"Ultrafast Dynamics in Aromatic Cation Based Ionic Liquids: A Femtosecond Raman-Induced Kerr Effect Spectroscopic Study"
Bulletin of the Chemical Society of Japan, 89, 1106-1128, 2016.

(14) T. Fujisawa, H. Kuramochi, H. Hosoi, S. Takeuchi, T. Tahara*
"Role of Coherent Low-Frequency Motion in Excited-State Proton Transfer of Green Fluorescent Protein Studied by Time-Resolved Impulsive Stimulated Raman Spectroscopy"
Journal of the American Chemical Society, 138, 3942-3945, 2016.

(13) T. Fujisawa, S. Takeuchi, S. Masuda, T. Tahara*
"Signaling-state formation mechanism of a BLUF protein PapB from the purple bacterium Rhodopseudomonas palustris studied by femtosecond time-resolved absorption Spectroscopy"
Journal of Physical Chemistry B, 118, 14761-14773, 2014.

(12) T. Fujisawa, M. Creelman, R. A. Mathies*
"Structural dynamics of a non-covalent charge transfer complex from femtosecond stimulated Raman spectroscopy"
Journal of Physical Chemistry B, 116, 10453-10460, 2012.

(11) H. Shirota*, H. Fukazawa, T. Fujisawa, J. F. Wishart
"Heavy atom substitution effects in non-aromatic ionic liquids: Ultrafast dynamics and physical properties"
Journal of Physical Chemistry B, 114, 9400-9412, 2010.

(10) T. Fujisawa, K. Nishikawa, H. Shirota*
"Comparison of interionic/intermolecular vibrational dynamics between ionic liquids and concentrated electrolyte solutions"
Journal of Chemical Physics, 131, 244519, 2009.

(9) H. Shirota*, T. Fujisawa, H. Fukazawa, K. Nishikawa
"Ultrafast dynamics in aprotic molecular liquids: A femtosecond Raman-induced Kerr effect spectroscopic study"
Bulletin of the Chemical Society of Japan, 82, 1347-1366, 2009.

(8) K. Osawa, T. Hamamoto, T. Fujisawa, M. Terazima, H. Sato, Y. Kimura*
"Raman spectroscopic study on solvation of p-aminobenzonitrile in supercritical water and methanol"
Journal of Physical Chemistry A, 113, 3143-3154, 2008.

(7) T. Fujisawa*, M. Terazima, Y. Kimura*
"Solvent effects on the local structure of p-nitroaniline in supercritical water and supercritical alcohols"
Journal of Physical Chemistry A, 112, 5515-5526, 2008.

(6) T. Fujisawa, T. Ito, M. Terazima, Y. Kimura*
"Raman spectroscopic study of the solvation of decafluorobenzophenone ketyl radical and related compounds in 2-propanol at ambient to supercritical temperatures"
Journal of Physical Chemistry A, 112, 1914-1921, 2008.

(5) T. Fujisawa, M. Terazima, Y. Kimura*, M. Maroncelli
"Resonance Raman study of the solvation of p-nitroaniline in supercritical water"
Chemical Physics Letters, 430, 303-308, 2006.

(4) T. Fujisawa, M. Terazima, Y. Kimura*
"Excitation wavelength dependence of the Raman Stokes shift of N,N-dimethyl-p-nitroaniline"
Journal of Chemical Physics, 124, 184503 (1-9), 2006.

(3) T. Fujisawa, M. Fukuda, M. Terazima, Y. Kimura*
"Raman spectroscopic study on solvation of diphenylcyclopropenone and phenol blue in room temperature ionic liquids"
Journal of Physical Chemistry A, 110, 6164-6172, 2006.

(2) 木村佳文、網田 富士嗣、藤澤知績
「超臨界流体中における非線形レーザー分光」
高圧力の科学と技術、日本高圧力学会、16巻2号、87-94、2006.

(1) Y. Kimura*, M. Fukuda, T. Fujisawa, M. Terazima
"Acceptor number of room temperature ionic liquid determined by the Raman spectrum of diphenylcyclopropenone"
Chemistry Letters, 34, 338-339, 2005.