Miyazaki, K., Kikukawa, T., Unno, M., Fujisawa, T. Cryo-Raman Observation on the Photocycle of a Light-Driven Cl– Pump from Mastigocladopsis repens at High Cl– Concentration.

J. Phys. Chem. B, 129, 3740-3746 (2025)

Miyoshi, R., Okuda, Y., Seto, R., Kamo, T., Fujisawa, T., Eki, T., Hirose, Y., Unno, M. Deprotonation at Ring B Is an Intrinsic Property of the Bilin Chromophore in Cyanobacteriochrome RcaE.

J. Phys. Chem. B, 129, 2986-2991 (2025)

Fujisawa, T., Tanaka, N., Tamogami, J., Unno, M. Retinal Chromophore Configuration in the O Intermediate of Sensory Rhodopsin II from Natronomonas pharaonis.

Biochemistry 63, 2714-2717 (2024)

Miyazaki, K., Kikukawa, T., Unno, M., Fujisawa, T. Chromophore Structural Change during the Photocycle of a Light-Driven Cl– Pump from Mastigocladopsis repens: A Cryogenic Raman Study

J. Phys. Chem. B 128, 9692-9698 (2024)

Kamo, T., Matsushita, T., Hamada, M., Fujisawa, T., Eki, T., Unno, M., Hirose, Y. Probing bilin-protein interaction in the protochromic photocycle of cyanobacteriochrome RcaE by site-directed mutagenesis. Plant Cell Physiol. 2024;, pcae085, https://doi.org/10.1093/pcp/pcae085

Nagae, T., Fujita, Y., Tsuchida, T., Kamo, T., Seto, R., Hamada, M., Aoyama, H., Sato-Tomita, A., Fujisawa, T., Eki, T., Miyanoiri, Y., Ito, Y., Soeta, T., Ukaji, Y., Unno, M., Mishima, M., Hirose, Y.  Green/red light-sensing mechanism in the chromatic acclimation photosensor

Sci. Adv. 10, eadn8386 (2024)

Machalska, E., Halat, M., Tani, T., Fujisawa, T., Unno, M., Kudelski, A., Baranska, M., Zając, G. Why Does One Measure Resonance Raman Optical Activity? A Unique Case of Measurements under Strong Resonance versus Far-from-Resonance Conditions

J, Phys. Chem. Lett. 15, 4913-4919 (2024)

Fujisawa, T., Unno, M. Near-Infrared Excited Raman Optical Activity as a Tool to Uncover Active Sites of Photoreceptor Proteins

J. Phys. Chem. B 128, 2228-2235 (2024) (Invited Perspective Article)

Fujisawa, T., Kinoue, K., Seike, R., Kikukawa, T., Unno, M. Configurational Changes of Retinal Schiff Base during Membrane Na+ Transport by a Sodium Pumping Rhodopsin
J. Phys. Chem. Lett. 15, 1993-1998 (2024)

Wu, T., Bouř, P., Fujisawa, T., Unno, M. Molecular Vibrations in Chiral Europium Complexes Revealed by Near-Infrared Raman Optical Activity

Adv. Sci. 11, 2305521 (2024)

Fujisawa, T., Shingae, T., Ren, J., Haraguchi, S., Hanamoto, T.,  Hoff, W. D., Unno, M. Spectroscopic Validation of Crystallographic Structures of a Protein Active Site by Chiroptical Spectroscopy

J. Phys. Chem. Lett. 14, 9304-9309 (2023)

Nishikawa, K., Kuroiwa, R., Tamogami, J., Unno, M., Fujisawa, T. Raman Optical Activity of Retinal Chromophore in Sensory Rhodopsin II

J. Phys. Chem. B 127, 7244-7250 (2023)

Ohya, M., Kikukawa, T., Matsuo, J., Tsukamoto, T., Nagaura, R., Fujisawa, T., Unno, M. Structure and Heterogeneity of Retinal Chromophore in Chloride Pump Rhodopsins Revealed by Raman Optical Activity

J. Phys. Chem. B 127, 4775-4782 (2023)

Fujisawa, T., Unno, M.

Advanced spectral analysis of complex molecular system

In Molecular and Laser Spectroscopy: Advances and Applications: Volume 3; Gupta, V. P. Ed.; Elsevier Inc.; pp 25-54 (2022)

Fujisawa, T., Kinoue, K. Seike, R., Kikukawa, T., Unno, M.

Reisomerization of retinal represents a molecular switch mediating Na+ uptake and release by a bacterial sodium-pumping rhodopsin

J. Biol. Chem. 296, 100792 (2022)

Okuda, Y., Miyoshi, R., Kamo, T., Fujisawa, T., Nagae, T., Mishima, M., Eki, T., Hirose, Y., Unno, M. Raman Spectroscopy of an Atypical C15-E,syn Bilin Chromophore in Cyanobacteriochrome RcaE

J. Phys. Chem. B 126, 513-821 (2022)

Fujisawa, T., Masuda, S., Takeuchi, S., Tahara, T. Femtosecond time-resolved absorption study of signaling state of a BLUF protein PixD from the cyanobacterium Synechocystis: Hydrogen-bond rearrangement completes during forward proton-coupled electron transfer

J. Phys. Chem. B 125, 12154-12165 (2021)

Fujisawa, T., Nishikawa, K., Tamogami, J., Unno, M.

Conformational analysis of a retinal Schiff base chromophore in proteorhodopsin by Raman optical activity

J. Phys. Chem. Lett. 12, 9564-9568 (2021)

Unno, M., Hirose, Y., Mishima, M., Kikukawa, T., Fujisaewa, T., Tamogami, J.

Spectroscopic approach for exploring structure and function of photoreceptor proteins

Biophys. Physicobiol. 18, 127-130 (2021)

Nagae, T., Unno, M., Koizumi, T., Miyanoiri, Y., Fujisawa, T., Masui, K., Kamo, T., Wada, K., Eki, T., Ito, Y., Hirose, Y., Mishima, M.

Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE

Proc. Natl. Acad. Sci. USA 118, e2024583118 (2021)

Kamura, S., Tani, T., Matsuo, H., Onaka, T., Fujisawa, T., Unno, M. New probe for porcelain glazes by luminescence at near-infrared excitation

ACS Omega 6, 7829-7833 (2021)

Akatsu, T., Fujisawa, T., Unno, M. Prestress in alumina-strengthened porcelain as estimated with Raman scattering spectroscopy

J. Ceram. Soc. Jpn. 129, 267-270 (2021)

Nakamizo, Y., Fujisawa, T., Kikukawa, T., Okamura, A., Baba, H., Unno, M. Low-temperature Raman spectroscopy of sodium-pump rhodopsin from Indibacter alkaliphilus: Insight of Na+ binding for active Na+ transport

Phys. Chem. Chem. Phys. 23, 2072-2079 (2021)

Matsuo, J., Kikukawa, T., Fujisawa, T., Hoff, W. D., Unno, M.

“Watching” a Molecular Twist in a Protein by Raman Optical Activity

J. Phys. Chem. Lett. 11, 8579-8584 (2020)

Fujisawa, T., Unno, M.

Vibrational optical activity spectroscopy

In Molecular and Laser Spectroscopy: Advances and Applications: Volume 2; Gupta, V. P. & Ozaki, Y. Eds.; Elsevier Inc.; pp 41-82 (2020)

Fujisawa, T.,

Raman Optical Activity of Proteins

Encyclopedia of Analytical Chemistry, 2020 John Wiley & Sons, Ltd.

Sato, T., Kikukawa, T., Miyoshi, R., Kajimoto, K., Yonekawa, C., Fujisawa, T., Unno, M., Eki, T., Hirose, Y.

Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome

J. Biol. Chem. 294, 18909-18922 (2019)

Fujisawa, T., Kiyota, H., Kikukawa, T., Unno, M.

Low-temperature Raman spectroscopy of halorhodopsin from Natronomonas pharaonis: Structural discrimination of blue-shifted and red-shifted photoproducts

Biochemistry 58, 4159-4167 (2019)

Iizuka, A., Kajimoto, K., Fujisawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Jung, K.-H., Unno, M., Demura, M., Kikukawa, T.

Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin

Sci. Rep. 9, 10711 (2019)

Osoegawa, S., Miyoshi, R., Watanabe, K., Hirose, Y., Fujisawa, T., Ikeuchi, M., Unno, M.

Identification of the deprotonated pyrrole nitrogen of the bilin-based photoreceptor by Raman spectroscopy with an advanced computational analysis

J. Phys. Chem. B 123, 3242-3247 (2019)

Takaoka, Y., Esaki, S., Sakaguchi, K., Fujisawa, T., Unno, M., Morisada, S., Ohto, K., Kawakita, H.

Size-dependent separation of graphene oxide by deformation of packed-gel in a chromatographic column

Sep. Sci. Technol. https://doi.org/10.1080/01496395.2019.1586726

Iwata, T., Nagai, T., Ito, S., Osoegawa, S., Iseki, M., Watanabe, M., Unno, M., Kitagawa, S., Kandori, H.

Hydrogen bonding environments in the photocycle process around the flavin chromophore of the AppA-BLUF domain

J. Am. Chem. Soc. 140, 11982-11991 (2018)

Fujisawa, T., Abe, M., Tamogami, J., Kikukawa, T., Kamo, N., Unno, M.

Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin

FEBS Lett. 592, 3054-3061 (2018)

Haraguchi, S., Shingae, T., Fujisawa, T., Kasai, N., Kumauchi, M., Hanamoto, T., Hoff, W. D., Unno, M.

Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration

Proc. Natl. Acad. Sci. USA 115, 8671-8675 (2018)

Fujisawa, T., Masuda, S.  Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins

Biophys. Rev. 10, 327-337 (2018)

Piao, W., Hanaoka, K., Fujisawa, T., Takeuchi, S., Komatsu, T., Ueno, T., Terai, T., Tahara, T., Nagano, T., Urano, Y.  Development of an azo-based photosensitizer activated under mild hypoxia for photodynamic therapy

J. Am. Chem. Soc.139, 13713-13719 (2017)

Fujisawa, T., Leverenz, R. L., Nagamine, M., Kerfeld, C. A., Unno, M. Raman optical activity reveals carotenoid photoactivation events in the orange carotenoid protein in solution

J. Am. Chem. Soc.139, 10456-10460 (2017)

Iwata, T., Nozaki, D., Yamamoto, A., Koyama, T., Nishina, Y., Shiga, K., Tokutomi, S., Unno, M., Kandori, H.Hydrogen bonding environment of the N3–H group of FMN in the LOV domains of phototropins

Biochemistry 56, 3099-3108 (2017)

Kajimoto, K., Kikukawa, T., Nakashima, H., Yamaryo, H., Saito, Y., Fujisawa, T., Demura, M., Unno, M.

Transient resonance Raman spectroscopy of a light-driven sodium-ion-pump rhodopsin from Indibacter alkaliphilus

J. Phys. Chem. B 121, 4431-4437 (2017) 

Kuramochi, H., Fujisawa, T., Takeuchi, S., Tahara, T.

Broadband stimulated Raman spectroscopy in the deep ultraviolet region

Chem. Phys. Lett. 683, 543-546 (2017)

Shin, N., Hanaoka, K., Piao, W., Miyakawa, T., Fujisawa, T., Takeuchi, S., Takahashi, S., Komatsu, T., Ueno, T., Terai, T., Tahara, T., Tanokura, M., Nagano, T., Urano, Y.

Development of an azoreductase-based reporter system with synthetic fluorogenic substrates

ACS Chem. Biol. 12, 558-563 (2017)

Furuta, M., Fujisawa, T., Urago, H., Eguchi, T., Shingae, T., Takahashi, S., Blanch, E. W., Unno, M.

Raman optical activity of tetra-alanine in the poly(L-proline) II type peptide conformation

Phys. Chem. Chem. Phys. 19, 2078-2086 (2017)

Shirota, H., Kakinuma, S., Takahashi, K., Tago, A., Jeong, H., Fujisawa, T.

Ultrafast dynamics in aromatic cation based ionic liquids: A femtosecond Raman-induced Kerr effect spectroscopic study

Bulletin of the Chemical Society of Japan, 89, 3942-3945 (2016)

Fujisawa, T., Kuramochi, H., Hosoi, H., Takeuchi, S., Tahara, T.

Role of coherent low-frequency motion in excited-state proton transfer of green fluorescent protein studied by time-resolved impulsive stimulated Raman spectroscopy

J. Am. Chem. Soc. 138, 3942-3945 (2016)

Haraguchi, S., Hara, M., Shingae, T., Kumauchi, M., Hoff, W. D., Unno, M.

Experimental detection of the intrinsic difference in Raman optical activity of a photoreceptor protein under pre-resonance and resonance conditions

Angew. Chem. Int. Ed. 54, 11555-11558 (2015)

Kimura, Y., Kasuga, S., Unno, M., Furusawa, T., Osoegawa, S., Sasaki, Y., Ohno, T., Wang-Otomo, Z.-Y.

The roles of C-terminal residues on the thermal stability and local heme environment of cytochrome c' from the thermophilic purple sulfur bacterium Thermochromatium tepidum

Photosynth. Res. 124, 19-29 (2015)

Zhao, W., Kirie, H., Tanaka, A., Unno, M., Yamamoto, S., Noguchi, H.

Synthesis of metal ion substituted P2-Na2/3Ni1/3Mn2/3O2 cathode material with enhanced performance for Na ion batteries

Mater. Lett. 135, 131-134 (2014)

Zhao, W., Tanaka, A., Tanaka, A., Unno, M., Yamamoto, S., Noguchi, H.

Synthesis of LiNi1/3Mn2/3O2 related cathode material from P2-Na2/3Ni1/3Mn2/3O2 for lithium ion battery

Mater. Lett. 134, 206-209 (2014)

Zhao, W., Kido, G., Harada, S. Unno, M., Noguchi, H.

Synthesis and characterization of anisotropically expanded graphite oxide compounds derived from spherical graphite

J. Colloid Interface Sci. 431, 8-16 (2014)

Urago, H., Suga, T., Hirata, T., Kodama, H., Unno, M.

Raman optical activity of a cyclic dipeptide analyzed by quantum chemical calculations combined with molecular dynamics simulations

J. Phys. Chem. B 118, 6767-6774 (2014)

Furuya, Y., Zhao, W., Unno, M., Noguchi, H.

The electrochemical properties of low-crystallinity TiO2(B)-carbon composite as an anode material in lithium-ion battery

Electrochim. Acta 136, 266-273 (2014)

Furuya, Y., Zhao, W., Iida, T., Unno, M., Noguchi, H.

Influence of preparation temperature of precursor on the thermal stability of TiO2(B) and its electrochemical property as an anode material in the lithium-ion battery

Electrochemistry 82, 7-13 (2014)

Kubota, K., Shingae, T., Foster, N. D., Kumauchi, M., Hoff, W. D., Unno, M.

Active site structure of photoactive yellow protein with a locked chromophore analogue revealed by near-infrared Raman optical activity

J. Phys. Chem. Lett. 4, 3031-3038 (2013)

Shingae, T., Kubota, K., Kumauchi, M., Tokunaga, F., Unno, M.

Raman optical activity probing structural deformations of the 4-hydroxycinnamyl chromophore in photoactive yellow protein

J. Phys. Chem. Lett. 4, 1322-1327 (2013)

Unno, M., Kikukawa, T., Kumauchi, M., Kamo, N.

Exploring the active site structure of a photoreceptor protein by Raman optical activity

J. Phys. Chem. B 117, 1321-1325 (2013)

Unno, M., Tsukiji, Y., Kubota, K., Masuda, S.

N-terminal truncation does not affect the location of a conserved tryptophan in the BLUF domain of AppA from Rhodobacter sphaeroides

J. Phys. Chem. B 116, 8974-8980 (2012)

Tateishi, Y., Abe, T., Tamogami, J., Nakao, Y., Kikukawa, T., Kamo, N., Unno, M.

Spectroscopic evidence for the formation of an N Intermediate during the photocycle of sensory rhodopsin II (Phoborhodopsin) from Natronobacterium pharaonis

Biochemistry 50, 2135-2143 (2011)

Nakao, Y., Kikukawa, T., Shimono, K., Tamogami, J., Kimitsuki, N., Nara, T., Unno, M., Ihara, K., Kamo, N.

Photochemistry of a putative new class of sensory rhodopsin (SRIII) coded by xop2 of Haloarcular marismortui

J. Photochem. Photobiol. B 102, 45-54 (2011)

Unno, M., Kikuchi, S., Masuda, S.

Structural refinement of a key tryptophan residue in the BLUF photoreceptor AppA by ultraviolet resonance Raman spectroscopy

Biophys. J. 98, 1949-1956 (2010)

Kikuchi, S. Unno, M., Zikihara, K., Tokutomi, S., Yamauchi, S.

Vibrational assignment of the flavin-cysteinyl adduct in a signaling state of the LOV domain in FKF1

J. Phys. Chem. B 113, 2913-2921 (2009)